Nicholas Raditya Putrawisesa

5 Questions 3 Answers 0 Followers

Questions related from Nicholas Raditya Putrawisesa

How to prepare acid phenol from saturated phenol solution?

Hi there. I'm planning to do an RNA extraction for seeds of the Arabidopsis plant. Currently, I have a saturated phenol solution (pH 6.6). How should I prepare the acid phenol solution from the...

08 March 2021 8,729 2 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

18 February 2021 7,809 4 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

03 February 2021 5,039 3 View

A260/A230 values are null?

Recently I did DNAse treatment and RNA clean up on some RNA samples I extracted from Arabidopsis leaves and flowers. After those treatment, I measure the concentration using a nanodrop...

19 February 2020 2,610 3 View

How do I find enzyme activity and specific activity at different concentrations of enzyme?

Dear Everyone, I'm doing experiment on finding enzyme activity and specific activity with different concentration of enzyme and same concentration of substrate. The enzyme comes from blood...

13 May 2019 2,455 3 View