Recently I did DNAse treatment and RNA clean up on some RNA samples I extracted from Arabidopsis leaves and flowers. After those treatment, I measure the concentration using a nanodrop spectrophotometer. During measurement, I notice that the value of A260/A230 in my samples were null. After checking the value of A230, turns of that the value is below zero. Aside from that, the value of A260/A280 in my samples are between 1.8 and 2.0. How this could happen? Should I redo the DNAse treatment? Before the treatment, I've checked my RNA samples concentration and all samples have A260/A230 and A260/A280 values.
Hi
Are you sure your blank solution is the same you used to dilute your samples AND that it is not contaminated with DNA/RNA?
I use DEPC-H20 as the blank solution, which I also used to dilute the samples. However, I think I will check whether the DEPC-H20 I used is contaminated or not
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