Nhu Y Nguyen

2 Questions 1 Answers 0 Followers

Questions related from Nhu Y Nguyen

DIfferent baseline start and end values in real-time PCR results among different samples on the same plate at the same run?

I am doing gene expression analysis using rt-qPCR (iTaq™ Universal 2X SYBR® Green Supermix). I use Applied Biosystems™ QuantStudio™ 3 real-time PCR machine. I ran my control and treatment rat...

23 November 2016 2,018 4 View

When there is a large variance in ELISA, what is the level of reliability of the assay?

In my case, during one experiment, I manually wash my plate three turns: 4 times per turn. And I experienced big differences in Absorbance (at 450nm) among triplicates. For example: Optical...

23 May 2014 1,043 5 View