Nesrine Sassi

5 Questions 3 Answers 0 Followers

Questions related from Nesrine Sassi

Gene Knockout efficiency ?

Hi everyone ! 'I've been working on a gene knockout in a BC cell line. The KO efficiency was explored for the first time after the limiting dilution and cell cloning by Western blotting. Some...

29 May 2025 5,137 1 View

Limiting dlution and single cell cloning ?

Hi everyone, I'm trying to do a limiting dilution and isolate one clone from T24 cancer cells after a gene knockout. I followed these steps: First, used a hemocytometer to quantitate the...

25 March 2025 3,142 3 View

CRISPR/Cas9 gene Knockout Troubleshooting ?

Hi everyone! I tried for the first time to do a gene Knockout in UMUC-3 cells. I'm using the ready designed plasmids from Santa Cruz Biotechnologie company, same for the transfection reagent and...

24 October 2024 8,922 2 View

Si RNA of NC causing Knockdown of the targeted gene ?

Hi all, I'll be explaining the situation briefly. I'm working with two cancer cell lines: T24 and UMUC-3 cells. I'm trying to do a knockdown for the SLC38A2 gene using siRNA and Hiperfect...

19 December 2023 4,299 0 View

In vitro macrophage infection with bacteria?

Hi everyone! I'm planning to do an in vitro macrophage infection with bacteria. I still do not know what kind of species I will be using, still haven't decided. those macrophages will be later...

28 October 2022 7,188 0 View