Nesreen Fayyad Al-Khuffash

5 Questions 3 Answers 0 Followers

Questions related from Nesreen Fayyad Al-Khuffash

How to prepare the following denaturation buffer: [2% SDS, 1 M β-mercaptoethanol (β-ME)]?

Hello, I hope analytical chemistry people or biochemistry major fellows could help me. Kindly advise on how to prepare the following denaturation buffer: [2% SDS, 1 M β-mercaptoethanol...

18 September 2023 3,920 3 View

What's the recipe for the "denaturation solution" for the endo-H sigma?

I want the home-made recipe of denaturation solution (5x per ug protein), for the "Endoglycosidase H - Sigma-Aldrich". Thank you in advance.

14 September 2023 705 0 View

What recommended protocol for Cycloheximide chase assay with transiently transfected Hek293T?

Hello Scientists, I am working on assaying the half life of a plasma membranal protein which is transiently transfected into Hek293T cell line using FuGene-HD transfection reagent. Kindly, if...

25 November 2021 2,335 1 View

What is the recommended ratio of each Na acetate:absolute ethanol used to purify plant RNA from contaminating polysaccharides & phenols?

RNA extraction from a desert plant is giving us a low concentration below the range. The main problem is that desert plant are really rich on polysaccharides, polyphenols and other various...

07 April 2015 4,280 12 View

House keeping genes as controls for CHX chase assay

I am looking for an explanation of why do we use house keeping genes (e.g: B-actin) as loading control. I read that it has a long half life that exceeds most proteins, but Still I found papers...

01 January 1970 9,795 1 View