Neeta Shrestha

3 Questions 4 Answers 0 Followers

Questions related from Neeta Shrestha

Can you advise me on the high efficiency transfection of the cells in the suspension?

I am working with the transformed bovine lymphocytes. These cells are in the suspension and I am planning to transfect these cells with the gene of my interest. Cell in suspension is often hard to...

13 February 2015 6,077 1 View

How can I prove quantitatively that all my cells are expressing GFP?

I have gfp expressing cells. Almost all of the cells are expressing GFP. But somehow the intensity of flourescence is very low and therefore it cannot be detected by FACS. Can someone tell me...

07 November 2014 4,766 5 View

What is the time point for the observation of global impact of viral miRNAs on transduced cells?

My experiment is related with miRNAs. I would like to see the effect of miRNA on transduced cells. So the idea is that miRNA after causing obstruction of protein production should show subsequent...

04 September 2014 1,559 3 View