Neelkant Verma

6 Questions 9 Answers 0 Followers

Questions related from Neelkant Verma

Does dye content of congo red affect the kinetics of binding to amyloids invitro?

Congo red comes in two variants one is >37% and second with >97% dye content, now question comes whether the dye content available affect the kinetics of binding hence giving false negative...

08 January 2018 4,420 2 View

Why ANS is settling at the bottom when incubated with HEWL?

ANS dye which is popular to study exposed hydrophobic patches in a protein structure, i have added it with hen egg white lysozyme but after few hours it gets settled down, what could be the reason...

28 November 2017 1,975 3 View

CD data is represented in ellipticity how can i convert it in to molar ellipticity?

generally we represent circular dichroism data in ellipticity (m.deg) but i want to represent it in to molar ellipticity, how can i convert it and represent in molar ellipticity manner?

31 October 2017 3,998 3 View

For lysozyme agrregation study which is the most suitable buffer?

i am working on lysozyme protein aggregation study (effects of several chemicals on its structure), i need suggestions for the buffer medium i should consider and why?

17 January 2017 553 6 View

Which fluorescence intensity measurement i should consider arbitrary units (AU) or counts per second (CPS)?

in fluromax instrument it shows cps and in varian eclipse its showing in AU

10 December 2015 7,707 5 View

Which is the most suitable protein extraction buffer for zebra fish brain tissue?

different buffers are reported in various journals, which is the most suitable one among them

10 August 2015 4,192 2 View