Why after CFSE staining, cells will die during 5 days of incubation, but not on the first day?

I stained one cryovial PBMCs according to the CFSE protocol (invitrogen C34570). One day before staining, I thawed the cells and rested them in media (ingredients is below ) for 24 hours. On the...

02 March 2023 4,613 2 View