In qPCR with TaqMan probes, can we distinguish primer dimer from true amplification of low-copy number of template RNA that come with high Ct values?

We do this for SARS-COV-2 detection from patients' swap samples. The virus is showing fluctuations in copy number in progress and there is no standard sampling, so we have trouble in deciding...

14 April 2020 4,106 2 View