Naz Rasiwala

3 Questions 3 Answers 0 Followers

Questions related from Naz Rasiwala

Immunofluorescent staining of thick (50-100 micron) cryosections?

Hello, I normally stain 12-micron cryosections for pancreas tissue (looking at insulin and glucagon expression). I would like to try staining 50 micron sections for confocal microscopy. Do I...

28 August 2021 4,518 0 View

Protocol for antigen retrieval on frozen sections (pancreas)?

I fix the pancreas tissues in 4% PFA for 24 hours, then put tissues in 30% sucrose until they sink. Then I embed the tissues in OCT and freeze them at -30. I am having trouble getting my...

15 April 2021 9,484 6 View

What is the optimal dose of tamoxifen by oral gavage in adult mice?

I am using a Cre-ERT mouse model and giving mice tamoxifen by oral gavage at 8 weeks of age. 1 dose of 6 mg of tamoxifen does not generate enough Cre activity. Does anyone have any recommendations...

18 October 2020 5,245 2 View