How can I prevent all the cells from emitting fluorescence in the red channel in a Live/Dead Assay of Microtissue?

I'm performing the viability Live/Dead assay on Microtissue (high cell density of cells - 250,000 cells/ mm3) using Calcein Am and Propidium Iodide and imaging them using a confocal microscope...

01 January 2014 8,682 13 View