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Questions related from Navaneethaiyer Umasuthan
I prepared 15% resolving and 4.5% stacking gels on the day before running SDS-PAGE and stored at 4°C with wet-paper towels and wrapped with cling wrap. Noticed the band broadening effect while...
02 February 2016 5,305 9 View
In an attempt to determine the transcriptional responses, I used three animals (at each time point) to conduct the experiment. I collected the tissues and POOLED the equal amount of tissues from...
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I was supposed to amplify a coding sequence of a target (~1.4 kb) to clone into an expression vector. Oligos were designed to flank the CDS (exactly from start to stop codons; Tm-59.5 C) with...
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I was using MatGAT for sequence similarity and identity calculations a while ago. However, recently I noticed running MatGAT on my computer repeatedly failed. MatGAT is a good tool to handle and...
09 September 2013 3,698 7 View
I have obtained 3 inter-related isoforms of lectins (~115 amino acids) with >80% of identity at amino acid level. Alignment shows only two regions of ORFs are sequence specific. To design qPCR...
06 June 2013 2,853 7 View