Navaneethaiyer Umasuthan

5 Questions 22 Answers 0 Followers

Questions related from Navaneethaiyer Umasuthan

How to solve the band broadening effect in SDS-PAGE?

I prepared 15% resolving and 4.5% stacking gels on the day before running SDS-PAGE and stored at 4°C with wet-paper towels and wrapped with cling wrap. Noticed the band broadening effect while...

02 February 2016 5,306 9 View

How may I justify my experimental design involving extracting RNA from POOLED tissues from three animals?

In an attempt to determine the transcriptional responses, I used three animals (at each time point) to conduct the experiment. I collected the tissues and POOLED the equal amount of tissues from...

05 May 2014 2,515 10 View

What has gone wrong in a cloning process where the sequence does not comply in the proximal region of the stop codon?

I was supposed to amplify a coding sequence of a target (~1.4 kb) to clone into an expression vector. Oligos were designed to flank the CDS (exactly from start to stop codons; Tm-59.5 C) with...

05 May 2014 3,489 7 View

Any similar tool to MatGAT to calculate the sequence similarity/identity (homology matrix)?

I was using MatGAT for sequence similarity and identity calculations a while ago. However, recently I noticed running MatGAT on my computer repeatedly failed. MatGAT is a good tool to handle and...

09 September 2013 3,701 7 View

Is there any negative impact in designing qPCR oligos in 3’- UTR region?

I have obtained 3 inter-related isoforms of lectins (~115 amino acids) with >80% of identity at amino acid level. Alignment shows only two regions of ORFs are sequence specific. To design qPCR...

06 June 2013 2,854 7 View