Nathaniel Ninyio

3 Questions 12 Answers 0 Followers

Questions related from Nathaniel Ninyio

My protein 16% alpha helix and 44% beta sheets but it gives the CD spectra for alpha helices. Is this normal?

I performed circular dichroism to estimate my protein sample's secondary structures. My sample buffer was 100 mM sodium phosphate (the CD of the buffer was measured and deducted from that of the...

07 July 2019 10,033 4 View

Does anyone know the sequence on the HBsAg that is the epitope to which the monoclonal antibody binds?

I performed western blot to detect the "a" determinant region of the HBsAg in my protein sample. This same protein has 6x his-tag. With anti-His antibody, I obtained two bands but with anti-HBsAg,...

08 August 2017 7,824 1 View

Please how can I insert a signal peptide in the vector I am to use for cloning?

I am to clone a chimeric protein gene sequence using the PFastBac HT C vector. However, I want to insert a signal peptide which will make my expressed protein to target the endoplasmic reticulum....

08 August 2017 3,045 16 View