Nathaniel Ninyio

5 Questions 12 Answers 0 Followers

Questions related from Nathaniel Ninyio

How many days or weeks after the final booster vaccine can I perform ELISPOT to check for memory B cells?

I please need to know how long I should keep vaccinated mice after administering the final booster dose before performing ELISPOT to check for the presence of memory B cells from their harvested...

14 January 2019 321 3 View

When assayed alone, is the B220 (CD45R) marker sufficient for immunophenotyping of memory B cells?

I want to perform immunophenotyping to check for a number of cell markers in the spleens of vaccinated mice. To check for memory B cells, I see that there are a number of cell markers. Because of...

11 January 2019 8,895 3 View

Please how can I insert a signal peptide in the vector I am to use for cloning?

I am to clone a chimeric protein gene sequence using the PFastBac HT C vector. However, I want to insert a signal peptide which will make my expressed protein to target the endoplasmic reticulum....

17 August 2017 9,824 14 View

How do I extract membrane proteins from Sf9 cells?

I am currently interested in identifying some receptor proteins on the plasma membrane of Sf9 cells. However, I find it difficult getting hold of any protocol that I can use to extract these...

29 March 2017 8,802 2 View

How do I set up a restriction digestion reaction when my DNA concentration is very low?

I want to carry out a single RE digestion using Thermo scientific's NcoI. The reaction protocol requires a DNA concentration of 1ug/uL in a 20uL reaction. My DNA concentration is 1ug in 21.3ul....

14 November 2016 9,551 5 View