Nathaniel Ninyio

3 Questions 11 Answers 0 Followers

Questions related from Nathaniel Ninyio

How many days or weeks after the final booster vaccine can I perform ELISPOT to check for memory B cells?

I please need to know how long I should keep vaccinated mice after administering the final booster dose before performing ELISPOT to check for the presence of memory B cells from their harvested...

14 January 2019 289 3 View

Please how can I insert a signal peptide in the vector I am to use for cloning?

I am to clone a chimeric protein gene sequence using the PFastBac HT C vector. However, I want to insert a signal peptide which will make my expressed protein to target the endoplasmic reticulum....

17 August 2017 9,789 14 View

How do I set up a restriction digestion reaction when my DNA concentration is very low?

I want to carry out a single RE digestion using Thermo scientific's NcoI. The reaction protocol requires a DNA concentration of 1ug/uL in a 20uL reaction. My DNA concentration is 1ug in 21.3ul....

14 November 2016 9,479 5 View