Nathan Kieswetter

2 Questions 4 Answers 0 Followers

Questions related from Nathan Kieswetter

What could be causing such low RNA yields from my TRIzol preps?

Hey All, So the goal of my latest set of experiments is to successfully extract great quality RNA from 1x10^6 cells (monocytes (THP-1) and macrophages(BAL washing's) using TRIzol Reagent for...

02 February 2016 9,408 5 View

At what concentration should THP-1 cells be frozen down at to encourage vigor and viability post thawing?

Hey All, I just wanted to check if anyone has observed differences (vigor and viability) in thawed THP-1 cells at varying initial freezing concentrations (Cell/ml). In general I freeze down...

04 April 2015 4,751 5 View