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Questions related from Nate Kove
Hello world, I'm trying to establish a growth curve for the P815s and I'm getting a doubling time of 6hr (it's the most consistent number I've found, but it's *not* very consistent). It does...
17 December 2019 8,752 0 View
Clearly I'm doing something wrong, and I'm sure it's a really silly mistake, but I can't figure out what it is. I'm using the easiest equation I could find which is from Wood et al 1977:...
16 December 2019 3,333 0 View
I'd like to infect an immortalized mast cell line with a virus, incubate for a day or two and then co-culture with purified CD8 T cells to induce antigen-specific activation. I see lots of...
10 October 2019 723 3 View
I'm wondering this for both, a general rule and more specifically for the flu.
03 October 2019 7,285 1 View
01 October 2019 4,832 3 View
From my understanding CD28 binds TCRs. However, if there are antigen-presenting MHCI cells also present in the culture media, would the CD28 compete with the antigen? So if I want an...
30 September 2019 3,191 5 View
I've only seen two ways to stimulate T cells: (1) coat surface with anti-DC3 and anti-CD28 (2) use bound (or beaded) anti-CD3 but free anti-CD28 Why isn't the CD28 ever free in suspension? Or...
09 September 2019 7,322 4 View
