Nate Kove

5 Questions 6 Answers 0 Followers

Questions related from Nate Kove

SRID calculation of titers gives unreasonable/impossible results?

Clearly I'm doing something wrong, and I'm sure it's a really silly mistake, but I can't figure out what it is. I'm using the easiest equation I could find which is from Wood et al 1977:...

16 December 2019 3,295 0 View

Can immortalized mast cell line (instead of splenocytes) serve as feeder cells for Tcell stimulation/expansion?

I'd like to infect an immortalized mast cell line with a virus, incubate for a day or two and then co-culture with purified CD8 T cells to induce antigen-specific activation. I see lots of...

10 October 2019 682 3 View

Can immortalized mast cell line (instead of splenocytes) serve as feeder cells for Tcell stimulation/expansion?

I'd like to infect an immortalized mast cell line with a virus, incubate for a day or two and then co-culture with purified CD8 T cells to induce antigen-specific activation. I see lots of...

01 October 2019 4,794 3 View

Do anti-CD28 abs compete with antigen-bound MHC during presentation to CD8s?

From my understanding CD28 binds TCRs. However, if there are antigen-presenting MHCI cells also present in the culture media, would the CD28 compete with the antigen? So if I want an...

30 September 2019 3,158 5 View

Why are T cells stimulated with bound CD3 but free floating CD28?

I've only seen two ways to stimulate T cells: (1) coat surface with anti-DC3 and anti-CD28 (2) use bound (or beaded) anti-CD3 but free anti-CD28 Why isn't the CD28 ever free in suspension? Or...

09 September 2019 7,281 4 View