Natalia Urbańska

3 Questions 5 Answers 0 Followers

Questions related from Natalia Urbańska

What - apart from popular reducing agents, e.g. b-mercaptoethanol or dithiotreitol, can cause disulfide bonds breakage?

Hello all, When performing SDS-PAGE electrophoresis under non-reducing conditions (without BME and DTT), disulfide bonds were broken. I am using a mPAGE 4-12% Bis-Tris Gel and MES buffer for the...

16 September 2021 9,775 6 View

What are EXPERIMENTAL inhibitors and activators of apoptosis?

Hello all, I am working on a project and I have to propose 3 methods to detect apoptosis, better than the one currently used in our facility. I have to cover the topic of activators and...

15 May 2021 1,474 8 View

How can I improve expression of phage protein in E. coli (BL21 DE3)?

I am working with tail tubular proteins from Y. enterocolitica-specific phages. These are small proteins, approximately ~20 kDa, they are fused with MBP, which gives 60 kDa. Transformation went...

19 November 2017 1,134 4 View