Nasrin Dorreh

3 Questions 2 Answers 0 Followers

Questions related from Nasrin Dorreh

Why does my Chromogenic Endotoxin Quant kit have high OD blank?

I am using this kit and facing high OD in my plate well that containing lysate buffer and substrate. I am using endotoxin free plate and pipette tips.

16 February 2025 4,573 1 View

What is the good dilution factor for mouse serum in ELISA?

I am doing IL6 ELISA to measure mouse serum IL6, but my ODs are so low even for mouse on two weeks high fat diet. My standard curve is good enough as a positive control.

29 April 2024 9,194 2 View

Could anyone provide me a complete protocol of IL6 mice plasma ELISA?

I've been measuring IL6 of mice plasma through ELISA but facing poor ODs for my samples, however standard curve and company controls are pretty fine.

14 July 2023 7,100 4 View