Why diifentiation media for 3T3-L1 cells doesnr arrest the growth?

I am trying to differentiate 3T3-L1 cells with using DMEM,FBS 10%, Insulin, IBMX and DEX. Althogh Ican see cells accumulating fat but they are still growing. I ve started differentiation with 70%...

13 November 2022 5,716 3 View