Nanfei Xu

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Questions related from Nanfei Xu

Why can't I re-amplify error-prone PCR products?

We have tried error-prone PCR with GeneMorph II Random Mutagenesis Kit and another kit from a Chinese supplier. Both kits worked fine in producing a specific product (single band on gel) of...

06 June 2015 8,243 3 View

Do I need codon optimization to express a plant gene in E. coli? If yes, what codon set should I use?

Trying to expression a plant gene for amino acid synthesis in E. coli, but couldn't detect the functioning of this gene in E. coli and couldn't see a band in protein gel. What is the likelihood...

03 March 2015 1,361 4 View