Namrata Chakravarty

3 Questions 8 Answers 0 Followers

Questions related from Namrata Chakravarty

Wrong sequencing result after cloning?

I have cloned a Bacillus gene in E.Coli pET20b vector at EcoRI and HindIII sites. The vector was already having a gene cloned at the same restriction sites, therefore, before ligating the bacillus...

04 July 2019 9,500 4 View

Why there is no expression of recombinant protein?

I have cloned a gene encoding an enzyme from gram positive bacteria in E.coli BL21(DE3), tried all the IPTG conc. and temperatures but there is no sign of expression. Is there any incompatibility...

04 August 2018 6,646 8 View

How to amplify a gene of a bacterial isolate in which the gene sequence is not known?

I have a bacterial isolate showing asparaginase activity but till now it is unreported and hence I am unable to find the sequence for asparaginase gene (although, the gene sequence is known in...

25 January 2018 5,566 3 View