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Questions related from Nagendra Verma
Hello to all I have little doubt about ssodna as a repair template for my Knockin experiment. Actually, I've designed 147nt long ssodna but I do not figure it out. whether I have to order an only...
28 August 2017 3,968 2 View
Hello, everyone, I want to create 6 SDM at a different location in the coding region using 6Kb Plasmid. I had chosen 3 different sites among 6 sites and started to mutate using QuikChange II XL...
25 July 2017 6,000 3 View
Hi.. Actually. I'm using the QuikChange II XL Site-Directed Mutagenesis Kit protocol based PCR program and I'm trying to transform in DH5alpha instead of XL-10Gold.Even I've been performed...
11 July 2017 6,523 9 View
Could anyone suggest me How can I design the scramble gRNA as a negative control to interpret my targeted gRNA for KO? Actually, I keen to know to the sequence composition of negative control...
30 May 2017 306 2 View
Could anybody suggest me Actually, I wanted biallelic deletion of 380bp sequences by using LentiCRISPRv2 vector. Is it Possible? If yes, I How many gRNA I suppose to design I order to haveing...
24 May 2017 1,827 1 View
Could anyone suggest me? Is it Possible to delete the 380bp sequence by using Lenti CRISPRv2 system? If Yes, How many gRNA I suppose to design order to have the biallelic deletion....
24 May 2017 4,986 1 View
Hi.. Everyone I have been attempted to Clone the Oligo sequence into the LentiCRISPRv2 backbone plasmid but I'm unable to get the colonies when I transformed in DH5alpha. I have followed the...
19 May 2017 7,006 1 View