Nagendra Verma

4 Questions 4 Answers 0 Followers

Questions related from Nagendra Verma

Can I transfect the only 147nt ssOdna 5'-3' (only sense strand)as repair template for Knockin experiment?

Hello to all I have little doubt about ssodna as a repair template for my Knockin experiment. Actually, I've designed 147nt long ssodna but I do not figure it out. whether I have to order an only...

28 August 2017 3,917 2 View

Hi..Every one Could any one suggest me about the mutagenesis PCR program and recipes?

Hi.. Actually. I'm using the QuikChange II XL Site-Directed Mutagenesis Kit protocol based PCR program and I'm trying to transform in DH5alpha instead of XL-10Gold.Even I've been performed...

11 July 2017 6,495 9 View

Hi. Everybody.. Could anybody has clue? How should I design the scramble sgRNA as a negative control for CRISPR Cas KO interpretation?

Could anyone suggest me How can I design the scramble gRNA as a negative control to interpret my targeted gRNA for KO? Actually, I keen to know to the sequence composition of negative control...

30 May 2017 276 2 View

Does anyone help me out to clone the Oligo sequence in Lenti CRISPRv2 backbone plasmid ?

Hi.. Everyone I have been attempted to Clone the Oligo sequence into the LentiCRISPRv2 backbone plasmid but I'm unable to get the colonies when I transformed in DH5alpha. I have followed the...

19 May 2017 6,974 1 View