Nagendra Verma

7 Questions 5 Answers 0 Followers

Questions related from Nagendra Verma

Can I transfect the only 147nt ssOdna 5'-3' (only sense strand)as repair template for Knockin experiment?

Hello to all I have little doubt about ssodna as a repair template for my Knockin experiment. Actually, I've designed 147nt long ssodna but I do not figure it out. whether I have to order an only...

28 August 2017 3,968 2 View

Does six substitution, PCR based mutation possible in the single gene at a different location using 6 Kb plasmid?

Hello, everyone, I want to create 6 SDM at a different location in the coding region using 6Kb Plasmid. I had chosen 3 different sites among 6 sites and started to mutate using QuikChange II XL...

25 July 2017 6,000 3 View

Hi..Every one Could any one suggest me about the mutagenesis PCR program and recipes?

Hi.. Actually. I'm using the QuikChange II XL Site-Directed Mutagenesis Kit protocol based PCR program and I'm trying to transform in DH5alpha instead of XL-10Gold.Even I've been performed...

11 July 2017 6,523 9 View

Hi. Everybody.. Could anybody has clue? How should I design the scramble sgRNA as a negative control for CRISPR Cas KO interpretation?

Could anyone suggest me How can I design the scramble gRNA as a negative control to interpret my targeted gRNA for KO? Actually, I keen to know to the sequence composition of negative control...

30 May 2017 306 2 View

Hi, Everyone. Could anyone suggest me? How should I KO the 380bp sequences by using the LentiCRISPRV2 plasmid?

Could anybody suggest me Actually, I wanted biallelic deletion of 380bp sequences by using LentiCRISPRv2 vector. Is it Possible? If yes, I How many gRNA I suppose to design I order to haveing...

24 May 2017 1,827 1 View

Hi, Everyone. Could anyone suggest me? How should I delete the 380bp sequences by using the LentiCRISPRV2 plasmid?

Could anyone suggest me? Is it Possible to delete the 380bp sequence by using Lenti CRISPRv2 system? If Yes, How many gRNA I suppose to design order to have the biallelic deletion....

24 May 2017 4,986 1 View

Does anyone help me out to clone the Oligo sequence in Lenti CRISPRv2 backbone plasmid ?

Hi.. Everyone I have been attempted to Clone the Oligo sequence into the LentiCRISPRv2 backbone plasmid but I'm unable to get the colonies when I transformed in DH5alpha. I have followed the...

19 May 2017 7,006 1 View