Nadia Gallenstein

4 Questions 5 Answers 0 Followers

Questions related from Nadia Gallenstein

Looking for protocol: mitochondria isolation from treated cell line with subsequent Analysis in Seahorse XFP (8well)?

Hey guys, i would like to know, if there is any existing protocol, how to isolate mitochondira from treated cells and use them further in Seahorse XFP Mito Stress test for 8well plates? Second...

10 October 2019 4,765 1 View

Why do bands disappear in western blotting?

We have samples from mouse visceral adipose tissue. The tissue was homogenized and lysed in icecold RIPA+ proteinaseinhibitors. The protein concentration was determined with BCA twice. We usually...

06 June 2019 1,232 8 View

Where do i get an average gene efficiency from for PCR analysis?

i would like to analyze a qRT PCR (SYBR green) with Real-time PCR Miner Version 4.0. Therefore I need the gene average efficiency, but i have no idea where to find it. The instructions say it is...

03 March 2019 8,346 3 View

How to choose a passage from cell line for experiments?

I am working with a cell line (EA.hy296, HUVEC hybrid) for the first time. How do I know, which passage is right to work with? I dont find any passages in publications except one thata says use...

06 June 2018 3,727 3 View