Nadia Gallenstein

6 Questions 9 Answers 0 Followers

Questions related from Nadia Gallenstein

MMP shedding in vesicles??

I read in several papers, that HUVECs (Shedding of the Matrix Metalloproteinases MMP-2, MMP-9, and MT1-MMP as Membrane Vesicle-Associated Components by Endothelial Cells;...

23 July 2021 3,804 3 View

Why does kaleidoscope Marker miss Bands in the middle??

We had several times the problem after the transfer: you can detect the yellow 10 kDa Band, the blue 20 kDa Band, the pink 25 kDa Band but after only the pink 75 kDa Band. 15, 37 and 50 kDa are...

03 February 2021 5,209 3 View

Endothelial Cell stimulation with LPS, Control is in IL6 and IL8 higher than stimulation?

I want to test a reagent and see if it has an inflammatory effect. Therefore I treated the Cells 5h with 1 µg/ml LPS as a positive control or left them untreated. However the untreated cells...

07 February 2020 5,585 3 View

Looking for protocol: mitochondria isolation from treated cell line with subsequent Analysis in Seahorse XFP (8well)?

Hey guys, i would like to know, if there is any existing protocol, how to isolate mitochondira from treated cells and use them further in Seahorse XFP Mito Stress test for 8well plates? Second...

30 October 2019 7,676 1 View

Can 3- Deoxyglucosone (3DG) form Methylglyoxal (MG)?

The two dicarbonyls are described to be highly metabolized in diabetic patients, linked to our sepsis studies. We first found extremely high plasma concentrations of MG in septic mice. Now we have...

19 September 2018 5,135 1 View

How to choose a passage from cell line for experiments?

I am working with a cell line (EA.hy296, HUVEC hybrid) for the first time. How do I know, which passage is right to work with? I dont find any passages in publications except one thata says use...

19 June 2018 6,167 4 View