Mwichie Namusamba

2 Questions 4 Answers 0 Followers

Questions related from Mwichie Namusamba

Why does HIF-1alpha in HUVECs treated with CoCl2 show two bands on WB?

Hi, I want to detect HIF-1 alpha in HUVECS treated with CoCl2 at 0, 50, and 100uM. I observed two bands at 100kDa (band #1) and 90kDa (band #2) in the treatments and the controls. However, the...

31 December 2020 9,757 1 View

How can I isolate cortical glial cells from the brain of wild type pups?

Hello, I am a PhD student in oncology. I am working on a project focused at exploring the brain tumor micro-environment. I want to experiment on conditioned medium from glioma cell line GL261...

21 December 2017 3,214 2 View