Mustafa Alsaraj

4 Questions 13 Answers 0 Followers

Questions related from Mustafa Alsaraj

Hi, I was doing recently western blotting, using Lamin A/C antibodies purchased fro NEB. The WB showing three size bands ..Any one knows why ?

For lamin A/C, two bands should appear only, but in my wb i get three and i cannot elucidate it.

01 January 2016 3,081 6 View

How long we can keep using set of primers? is freeze-thawing cycle as we take them from the freezer affects its efficacy?

I have set of primers, in my stock stored at -20C, but I wonder how long I can keep use them? Thanks

12 December 2015 1,141 9 View

How to keep my RNA samples nuclease-free?

Hi, I do alot of work related to RNA, from Extraction to making cDNA library to loading agarose gel with samples of PCR products. Are there any roles or advice to minimise RNA exposure to nuclease...

12 December 2015 1,461 2 View

I run PCR products in a gel, however, out of 10 samples I get 2 missing, primers are validated and RNA integrity is v.good, any idea how to fix it?

I used to run gel before with no problems, but lately I started to get samples missing. I do prepare new TBE buffer everytime I ran a gel, but this seems not to be the problem, since I get other...

12 December 2015 2,274 9 View