Mustafa Selim Dogru

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Questions related from Mustafa Selim Dogru

Which washing solution is best in chemiluminescens probing in western blot? PBS /PBS-Tween 20 or TBS/TBS-Tween 20?

I am working with chemiluminescence substrate in western blot. Some of researchers suggest PBS (phospate buffered-saline)/PBS-Tween 20 for washing the membran after blocking and primary/secondary...

29 August 2016 5,671 8 View

What type of primary antibody do you suggest for western blotting?

What kind of antybodies (for COX-2, Bax, Bcl2, NfKB p65, p53) do you prefer for western blotting (target reactivity specie is rat) : 1. Plyclonal or monoclonal ? 2. Rabbit or mouse ? 3. Which...

18 May 2015 9,340 4 View