Muntasir Alam

7 Questions 14 Answers 0 Followers

Questions related from Muntasir Alam

What are the challenges during isolation of Candida spp from vaginal swab?

I am preparing a protocol on Isolation of Candida spp from vaginal swab. At this moment, we don't have differential CHROMagar Candida medium in hand. We have blood agar, chocolate agar, general...

25 January 2020 7,810 14 View

What is the most reliable commercial ELISA kit for detection of HIV-1 gp120?

I want to detect gp120 shedding from cell surface and virion. Therefore, I am looking for an reliable commercial ELISA kit. Please suggest.

09 August 2016 4,057 3 View

Is it mandatory to use 5`-phosphorylated primers in site directed mutagenesis?

I'm try to perform site directed mutagenesis to introduce point mutation in my desired gene. So far I have failed to see any colony. I'm following in house method of liner amplification by pfu...

10 November 2014 5,793 8 View

Site directed mutagenesis kit: Which one is most satisfactory?

I have a gene of interest (HIV-1 envelope) inserted into a plasmid. Now I want to insert specify nucleotide substitution in some point by site directed mutagenesis. Please suggest me some good...

05 August 2014 5,039 5 View

What is a good plasmid for HIV-1 envelope expression vector?

I need to check the binding of antibodies against HIV-1 Envelope expressed on cell surface (293T). I have several mutant HIV-1 envelope in a plasmid which do not express EGFP. So, I need to clone...

28 April 2014 7,353 5 View

Protein-peptide docking Software?

I have a PDB file for viral envelope and an inhibitor peptide. Now I want to see the binding of that peptide with viral envelope. Which software to use? Can anyone suggest something easier?

10 October 2013 2,761 9 View

How to efficiently amplify the entire env gene (envelop gene) of HIV-1 clade B from a plasma sample?

I want to first amplify the HIV-1 env gene from a patient's plasma samples and then I want to clone it into a plasmid vector. I am using the QIAamp viral RNA mini kit for the extraction of HIV-1...

17 January 2013 6,638 12 View