Munawar Ali

5 Questions 5 Answers 0 Followers

Questions related from Munawar Ali

How can I extract pure genomic DNA from dry plants?

I extract DNA from different parts of dry herbs like dry leaves, seeds, stem, roots, seed coat, rhizome flowers, fruits etc via CTAB method and then I use that DNA for PCR amplification. I face...

10 October 2019 4,508 3 View

I am not able to obtain PCR results for matK & rbcL. what could be the possible reason is there any problem with my Temp DNA or PCR?

I extracted total DNA of Hedera helix plant's leaves via CTAB method then i used to amplify the matK & rbcL genes at different PCR protocols (Temperature and time) the reaction mix used is Master...

08 August 2019 1,386 14 View

What is the best suitable protocol of performing plant extraction and filtration for conducing antimicrobial activities?

I need to know the best suitable protocol of performing plant extraction via ethanol, methanol, water and chloroform etc. I would also like to know that what size and type of filter should be used...

07 July 2018 4,956 0 View

How many and which primers of rbcL gene would be used for sequencing of PCR amplicon obtained from the R & F primers of rbcL gene for DNA barcoding?

I am going to dispatch PCR amplicon of my plants obtained from the rbcL primers for sequencing. Someone told me that it will not only need the Rev: & Fwd: primers but will also need a third...

01 January 2018 8,554 1 View

Can anyone provide the complete Micropropagation / Tissue Culture protocol of Gerbera Double hybrid plants on commercial scale?

Tissue Culture of Gerbera double Hybrid. What part should be used as Explant? Which media and hormones should be used? how long will it take to raise plants via Micropropagation?

01 January 1970 1,061 6 View