Mukti R. Paudel

3 Questions 8 Answers 0 Followers

Questions related from Mukti R. Paudel

How I select the antibodies for the western blot analysis of some secondary metabolites isolated from plant extract on HeLa and U-251 cell lines?

Secondary bioactive compound groups were isolated from the Dendrobium orchid methanol extracts by chromatographic techniques. Each group has up to 5 pure compounds. I am confused for the selection...

11 September 2017 4,922 0 View

How to Calculate MIC value?

I had used 3 different concentrations of plant extracts for different bacteria using ciproflaxacin as positive control. I had measure the zone of inhibition of extracts as well as control.

14 January 2016 5,424 23 View

How can I calculate cell survival rate or cell inhibition percentage for the calculation of IC50?

I had used MTT assay for cytotoxicity and used treated cells with MTT plus DMSO as sample test, untreated cells with MTT plus DMSO as control and no cell (only medium) with MTT plus DMSO as blank.

02 January 2016 2,680 10 View