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Questions related from Muhmmad Sajid
Hi, i want to produce a high titer of lentivirus using psPAX2, pVSVg, and third plasmid (my GOI cloned) but I am confused about what molar ratio will be best to get higher titer of lentivirus. i...
16 November 2023 9,302 2 View
Hi all I am working on immortalized human keratinocytes N/TERT-1 cells and trying to produce KI and KO cell lines by CRISPR/CAS9 system. i successfully got the single clones but when i tried to...
03 February 2023 6,777 1 View
Hi all. I have to ask a question. I checked inhibition of my gene to in in vitro And got a good results. but when I tried to prove it in vivo I didn’t get the inhibition of virus by the gene. I...
18 August 2020 1,289 0 View
i am using ChIP assay for the binding of nucleic acid and protein. i used normal mouse IgG as a negative control, Anti polymerase II as a positive control and specific ChIP grade antibody for my...
18 May 2020 5,409 0 View
Hi i am using HepG2 cells recently i encountered a problem. HepG2 cells contains small dots which sometimes affect the growth of cells or sometimes don't affect as can seen in pictures. media...
16 November 2019 6,079 4 View
