Muhammad Zahoor

5 Questions 9 Answers 0 Followers

Questions related from Muhammad Zahoor

How to tranfect Cas9 (RNP) protein into Hela cells using lipofectamine, crispmax or Mirus?

does any one have protocol for the effecient transfection of Cas9 Protein (RNP) along with gRNA in Hela Or U2OS, MC3T3-L1 cells using lipofectamine 2000, Mirus etc. thank you in advance.

10 October 2019 1,454 2 View

Any one have experienced with western blotting for Hif1a?

Hi, i am having trouble with immunoblotting for HIF1a, and would like to have some tips from you guys. i have tried 4-12% SDS gel, dried/fast and wet transfer both, using Anti-Hif1a from R&D...

08 August 2016 1,668 13 View

DAB kit for goat primary ab?

Hi, i am interested to do immunohistochemistry (DAB staining) on mouse tissue, and looking if some one can suggest me any good kit. I do have primary antibodies from goat against the protein i am...

12 December 2015 4,099 4 View

How to seperate or purify the double allele KO cells from single allele KO cells, using CRISPR/Cas9?

I am using CRISPR/Cas9 system to KO a gene and the plasmid carries GFP as label. However, I am not sure how would I be able to differentiate or purify the double allele KO cells from single allele...

06 June 2015 2,383 8 View

Are you familiar with the HUVEC cells tube formation assay?

I am interested to perform HUVEC cells tube formation assay. Currently I am culturing HUVEC cells in Medium200+LSGS and can I use the same medium for tube formation assay? Please suggest a...

04 April 2015 2,235 3 View