Muhammad Zain Ul Arifeen

3 Questions 6 Answers 0 Followers

Questions related from Muhammad Zain Ul Arifeen

What instrument can I use to monitor oxygen concentration in anaerobic glove chamber?

I am working with anaerobic chamber to test some fugal species, isolated from sub-seafloor (2.5 km) below the seafloor, in anaerobic chamber and now I am facing a problem as non of the specie is...

06 June 2018 8,630 4 View

How to ensure that the target DNA after cutting with RE will ligate to correct position on plasmid also cutted by same RE?

Assume that the cloned fragment DNA and plasmid have the same cuts by identical restriction enzyme, for instance, Bam HI, is it correct that the foreign DNA could be inserted in a fixed direction?...

10 October 2017 4,383 8 View

What is the best buffer system for cutting DNA fragment before cloning?

Before cloning, a DNA fragment needs double digestion to create two cohesive ends, thus two different enzymes are required. If the best performance of these two enzymes varies in different buffer...

10 October 2017 493 3 View