Muhammad Abdul Samad Naqvi

3 Questions 2 Answers 0 Followers

Questions related from Muhammad Abdul Samad Naqvi

Can mammalian DNA-PK inhibitors used for the Inhibition of NHEJ to improve the CRISPR knock-in efficiency ?

I want to make a CRISPR knock-in S2 cell line, but efficiency is too low. I have figured out different ways to achieve it as: S2 cells synchronization with cholechicine RNase H II use in...

14 August 2024 8,537 0 View

Ptimal colchicine concentration and time for S2 cell cycle arrest? Incubation duration in serum-free media for S2 cell cycle arrest?

Hi, I am seeking advice on the optimal conditions for synchronizing Drosophila S2 cells. Specifically, I am interested in determining the ideal concentration of colchicine required to effectively...

26 July 2024 5,753 0 View

Arabidopsis plant mutants grew on +P and -P minimal media but seeds have turned into black and germination is not normal Kindly suggest reason?

Plant genetics

26 March 2024 1,124 1 View