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Hello, I am planning to administer a statin drug to mice injected with a leukemic cell line overexpressing a chemokine gene to test the antileukemic effect of the statin treatment. I checked and...
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why the frequency of CRISPR-Cas9 mediated HR is always more than the normal HR in a cell/bacteria?
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I want to knock out a gene using CRISPR, to design the arm do I need to replace the whole CDS by a marker gene (GFP) or just induce a frame shift mutation? please suggest.
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I want to insert EGFP gene into MTb genome using CRISPR system. Is it right to design gRNA targeting an essential gene and HDR template (1.5kb)? I design is just the last 400 bp of one gene1 with...
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