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I prepared deionized water through deionizer, but unfortunately the pH dropped to 5. Now how can i resume the pH to 7, to use this water in PCR and other solutions preparation?
25 April 2018 2,722 6 View
I have prepared lysis buffer containing tris HCl, EDTA and SDS what is the best temperature to prevent it from pH shifting and reuse it. Containing 50mM tris HCL 20mM EDTA 10mM Nacl 2% SDS.
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I am using a protocol which have DTT but some people have extracted DNA without DTT, and some says keratine cannot be digested without it. As DTT is very expensive is it possible to extract DNA...
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