Muhammad Behroz Naeem Khan

4 Questions 10 Answers 0 Followers

Questions related from Muhammad Behroz Naeem Khan

What's the best way to detect the extracted lipids in order to confirm that your lipid extraction protocol is correct?

I am extracting Lipids from RBC Membranes using the Cholorform/Methanol (2:1) method. After phase separation and consequent evaporation of the organic phase, what would be the best way to confirm...

03 December 2019 8,108 2 View

DNA Quantification on Thermoscientific Multiskan Go?

I quantified the extracted DNA on Multiskan Go. The provider of the instrument directed me to take the absorbance at 260 and multiply it by 1000. According to him this would give the concentration...

29 March 2018 2,787 2 View

Can I perform histopathology on cell lines?

I have transfected cell line with an inhibitor of a particular gene. that gene promotes tumor growth. Now I want to check whether the inhibitor has caused change in the tumor growth. So can I do...

26 February 2018 209 6 View

Micro RNA expression profiling

I have isolated total RNA from blood samples of HBV by trizol method, converted it into cDNA and will now perform Real Time PCR for expression analysis of selected miRNAs by using the specific...

31 May 2017 2,593 4 View