Muhammad Husnain Ahmad

6 Questions 5 Answers 0 Followers

Questions related from Muhammad Husnain Ahmad

What is the difference between TUNEL and DAPI staining?

In the detection of programmed cell death, the literature shows by using Promega PCD kit, the TUNEL staining shows green fluorescence and DAPI staining shows blue fluorescence. I want to make it...

24 July 2023 7,848 9 View

What is the working concentration of DAPI?

Can anyone recommend the working concentration of DAPI for staining pollen cells

04 July 2023 2,311 2 View

How to add fluorescent dye in cultured pollen cells and subsequently wash?

Hello researchers. In my pollen culture experiment, I have to add protein, stain the pollen tubes with fluorescent dye and wash 2-3 times before imaging. During this washing step with the washing...

24 May 2023 9,630 0 View

Should the Alpha value be set to 5% or 1%?

Hello Researchers I am wondering that under what conditions it is more suitable to use an alpha value of 1% instead of 5% (using t-test). I have some data. When I use alpha value of...

08 March 2022 7,449 4 View

Phospholipid is not getting soluble?

In my experiment, I want to use phospholipid (Phosphatidic Acid) in my cell culture media. According to protocol, I soluted Phosphatidic Acid (PA) in chloroform then later diluted in culture...

19 November 2021 5,752 3 View

How to make solution of phosphatidic acid?

Hello researchers I am working on cell culture. I have to make solution of phosphatidic acid. Anyone can guide me how to make its solution in chloroform (solubility of phosphatidic acid) and later...

12 September 2021 1,810 0 View