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Questions related from Moustafa Gabr
I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...
01 November 2018 1,124 2 View
I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...
08 October 2018 3,967 0 View
I did find few examples of using ITC studies to investigate binding mode of small molecules with amyloid fibrils (using insulin fibrils and Ab-40) but could not find any with Ab-42 which is more...
09 November 2017 3,191 2 View
I am wondering if there is any explanation for the dependence of solubility of Co(II) inorganic compounds in water on the counter ion. In particular, cobalt (II) cyanide is completely insoluble in...
22 September 2017 8,268 8 View
Are there any well established fluorescent dyes (alternatively to PicoGreen) that can differentiate between supercoiled and relaxed DNA, so that they are able to measure the difference in...
11 July 2017 5,848 2 View
I am running a Stille coupling using an excess of 2-tributylstannyl pyridine and having a hard time purifying my product because of the tin byproduct and the excess reagent. I have tried washing...
21 June 2017 465 4 View
Tuning luminescence of platinum complexes is well established by variation of cyclometalating ligand. The most common trend I find in literature is a red shift in the emission upon introducing...
09 June 2017 4,277 3 View
I have been using Swissdock and BiosolveIT for molecular docking recently. However, I have an issue when I try to do the modelling for metal complexes (particularly Rhenium complexes). Both don't...
18 April 2017 7,380 14 View
Can anyone provide a protocol for AFM liquid sample preparation of protein?. My sample is amyloid beta aggregates in PBS.
20 January 2017 5,440 5 View
I am solving crystal structure with Olex2 (using ShelXL settings). The structure solves well to R value 2.95% but during the refinement one carbon atom in a thiophene ring in my structure shows 2...
30 November 2016 477 4 View
I am using ThT and Congo red assays to monitor Amyloid Beta 1-42 aggregation. It worked fine before, but now I am evaluating compounds with overlapping UV absorabance to these dyes. So any...
11 November 2016 3,497 7 View
Is there a way to selectively reduce alpha, beta unsaturated aliphatic ketone in presence of aromatic ketone? would Luche conditions achieve that ? Thanks
24 January 2016 5,619 3 View
I want to selectively reduce secondary amide in my compound in presence of nitro and Boc groups
17 December 2015 6,341 3 View
