Mouna Aref Mikati

2 Questions 3 Answers 0 Followers

Questions related from Mouna Aref Mikati

Yellow floating mass in cell cultures

Hello, We have just started growing cryopreserved primary human epithelial keratinocytes provided from Thermo Fisher, and three days after culture initiation we noticed a yellow floating mass in...

01 August 2017 313 3 View

If I plate cells from ones that were cryopreserved at the end of the primary culture, would the cells I am plating be considered P0 or P1?

I would like to understand how to correctly determine the passage number of the cell cultures we are growing. Our lab currently purchases cryo-preserved cells that were frozen at the end of the...

14 June 2017 6,657 4 View