Motolani Matthew

2 Questions 3 Answers 0 Followers

Questions related from Motolani Matthew

How do I use Hydrogen-deuterium exchange mass spectrometry to measure the folding and unfolding rates of protein?

I have used HDX-MS to measure the stability of staph nuclease at different time points exchange times, but I am looking to extract the rates of folding information from my results. I have used the...

01 June 2022 9,919 0 View

Should I subtract blank when determining protein fraction unfolded by fluorescence spectroscopy?

I am using fluorescence spectroscopy to determine stability parameters for human acidic fibroblast growth factor 1. (FGF1). The fraction unfolded for FGF1 is determined from the ratio of emission...

25 January 2022 9,390 2 View