Mostafa Khedrinia

6 Questions 6 Answers 0 Followers

Questions related from Mostafa Khedrinia

Introducing buffers with low EC for purification?

I need a buffer with an EC less than 0.2. Can someone introduce me to such buffer for purification by HIC method.?? Thanks

18 January 2024 1,256 0 View

Buffer preparation for hic chromatography method?

I'm purifying a recombinant protein. Currently, I use HIC to purify it. The specification of my Bind and Elution buffer is according to the following concentration, EC and pH... Bind, Sodium...

16 January 2024 8,120 2 View

Does protein denaturation increase its hydrophobicity??

Hello. What is the correlation between protein denaturation and its hydrophobicity? What is the effect of changing the hydrophobicity on retention time (reverse phase chromatography)? in my...

18 November 2023 4,135 1 View

How to prevent dimer and trimerization of my protein during refolding?

Hello, I am working on a recombinant protein named Brolucizumab. the protein is designed as a monomer with a molecular weight of 26kd. It has 4 cysteines that form 2 s-s bind. I have a problem...

13 November 2023 9,516 6 View

Method for Washing the inclusion body?

I use the following method to wash my inclusion bodies: 8 steps. 1: Troton, EDTA, Tris and Fresh DTT (3 Times) 2: Doc, EDTA, Tris and Fresh DTT (3 Times) 3: PBS 4: Water I use this method for 3...

25 September 2023 857 2 View

The reason for precipitation or aggregation of my protein in the refold buffer?

I use the refolding buffer with the following specifications for the refolding of brolucizumab protein. l-Argenine, Sorbitol, EDTA, Tris, and fresh Cystein and Cistine. I have already used these...

11 September 2023 4,477 2 View