Moin Chowdhury

4 Questions 3 Answers 0 Followers

Questions related from Moin Chowdhury

How to remove gapped plasmids from nicked plasmids?

I have a plasmid sample (10kb) with two different isoforms: dsDNA nicked dsDNA with a single-strand gap (at least 10 bases) I want to make sure my sample has only nicked plasmids, no plasmids...

03 March 2019 7,592 3 View

How can I confirm that my CRISPR mutants have homozygous and not heterozygous mutations?

TL;DR: I think I had a PCR/Sequencing artifact fool me into thinking I had a homozygous deletion mutant, where it may actually be heterozygous (or vice versa). I want to know how to avoid this....

12 December 2018 4,323 2 View

What conditions are optimal for growing RPE-1 colonies from single cells?

I've made a few attempts at flow cytometry using RPE-1 cells (ATCC), but have been getting issues with colony formation. This problem exists even for untransfected cells at the slowest sorting...

12 December 2017 8,798 0 View

Have you ever had a 0.25mm vertical sequencing gel run with horizontal streaking?

I'm wondering what the cause might be. This is a DNA sequencing gel run with 0.25mm spacers and running at 2000V. I don't understand why I would get streaking like this at all. Is it possible that...

02 February 2014 1,078 2 View