Mohan Govindasamy

3 Questions 3 Answers 0 Followers

Questions related from Mohan Govindasamy

What is the best way to use BLAST to search for homologous sequences to avoid off-target effects while selecting cDNA sequences for dsRNA in RNAi exp?

I have selected around 400bp of cDNA sequence for making dsRNA for RNAi experiment. I have used normal BLAST search for checking sequence homology to avoid off-target effects. I want to know what...

16 August 2018 349 2 View

How to lyse bacteria to extract dsRNA using Trizol?

I need to extract dsRNA from a dsRNA expressing bacteria by using Trizol. I want to know how to properly lyse the cells. If you have full protocol for extracting dsRNA in bacteria using Trizol...

06 June 2017 381 4 View

Is there any way to get individual dTTP from oligo dT?

I want dTTP , since I have oligo dT with me for making reverse transcription. I want to know is there any way to obtain individual dTTP from oligo dT.

23 March 2017 1,151 4 View