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I have been doing my thesis on real time PCR but I can't draw my standard curve, the points are too far from the slope. I have checked my primer conc through gardiant , syber green, cDAN, Tm. But...
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ı would be grateful, if you gave more detail information about it.
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Is there any known tool to find the promoter region's of any gene?
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my background is Plant Sciences.
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