Mohammed Abdelbaset

4 Questions 1 Answers 0 Followers

Questions related from Mohammed Abdelbaset

If I removed the Plasmid ATG to prevent tag expression, the expression would start at ATG of my desired protein?

I am using pET-51b vector and I do not want the tags to be expressed with my gene. so I want to design primer to remove Plasmid ATG: 1- ATG (plasmid)-tag_ ATG (desired gene) 2- (plasmid without...

12 December 2019 1,908 4 View

Can the insert gene leave the plasmid after cloning?

after doing succefel ligation and transformation to Ecoli BL21, I made glycerol stock. Then when I retured, I found the plasmid without the insert How is this possible?

10 October 2019 8,718 4 View

How to carry out assay for activity of non-purified Carbonic Anhydrase ?

I expressed CA in E-coli and I want to make sure the expressed protein is carbonic anhydrase, is there any test to determine the activity of non purified CA ? Thanks in advance

03 March 2019 8,165 1 View

After chlorosulfonation of aniline, three positions were attacked two ortho and para. How to select these attacks in one ortho and para positions?

After chlorosulfonation of aniline, three positions were attacked two ortho and para. How to select these attacks just in one ortho and para positions? The reaction temperature was 0 Cand then...

08 August 2017 9,768 1 View