Mohammad Zulkifli

3 Questions 3 Answers 0 Followers

Questions related from Mohammad Zulkifli

Fast and reliable method to determine the NADP/NADPH ratio in yeast cells ?

I want to determine the NADP/NADPH ratio in yeast cells. Any suggestion which method/kit work best with yeast cells. Thanks

07 July 2018 8,902 0 View

How to look for genetic interaction between two genes of interest with one being an essential gene in the yeast, Saccharomyces cerevisiae?

Hi, I want to check genetic interaction between two genes in Saccharomyces cerevisiae by making a double deletion and look for the synthetic sick or lethal phenotypes on non-fermentative carbon...

01 January 2017 2,276 4 View

Problem in cloning yeast gene under native promoter using Dh5alpha E coli cells - Can you lend your expertise?

I am trying to clone a gene from yeast under native promoter in pRS 316vector in DH5 alpha. PCR working , digestion working but no colonies on Amp plate after ligation. I got positive clones for...

07 July 2014 3,143 4 View