Mohammad Salik Zeya Ansari

2 Questions 10 Answers 0 Followers

Questions related from Mohammad Salik Zeya Ansari

Why are my cells (RAW 264.7) are not adhering on culture plates when I was reviving the cell?

I had frozen my RAW 264.7 cells from T-75 flask having 80-85% confluency, washed with 3ml PBS respectively. After washing, I added 3ml of fresh media and scrapped all the cells (also, could we use...

10 October 2018 1,849 5 View

My transfected cells started dying after sixth days of transfection?

I had transfected Ovarian Cancer cell line along with pCMV6-AN-HA tag vector along with my GOI POTEB in PD60mm. I have seen expression within 24 hrs after infection and it shows >90%...

02 February 2018 2,303 5 View