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I had frozen my RAW 264.7 cells from T-75 flask having 80-85% confluency, washed with 3ml PBS respectively. After washing, I added 3ml of fresh media and scrapped all the cells (also, could we use...
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I had transfected Ovarian Cancer cell line along with pCMV6-AN-HA tag vector along with my GOI POTEB in PD60mm. I have seen expression within 24 hrs after infection and it shows >90%...
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