Mohammad Wasiful Gofur

5 Questions 3 Answers 0 Followers

Questions related from Mohammad Wasiful Gofur

Books or websites to learn about different enzymes and kits?

As a PhD student, while optimizing an RNA-seq protocol, I am fascinated to learn about different enzymes and kits as well as the exciting ways to utilize them. This encouraged me to develop my...

10 July 2024 4,314 0 View

What are these large DNA fragments (1200 bp) after PCR using a cDNA template synthesized by Maxima H Minus Reverse Transcriptase?

Hi, I am using VASA-seq for RNA sequencing. The last two steps of the protocol are cDNA synthesis (via reverse transcription) and PCR. I had been using Superscript III for cDNA synthesis and was...

10 March 2024 6,947 2 View

The PCR step of VASA-seq is not working. Does anybody have any idea why?

We're trying to establish VASA-seq, an RNA sequencing protocol (Salmen et al. 2022, Nature) in our lab. Although having a sufficient amount of amplified RNA (around 100 ng/ul) after in vitro...

10 January 2024 2,804 0 View

Statistical test and graph to show the correlation of the expression pattern of two target genes at different time points?

I have the RT-qPCR data (CT) of two different target genes measured at different time points along with a housekeeper gene. I have calculated the 2^(-ddCT) values. Now I wonder how I can check if...

09 April 2021 6,253 1 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

21 February 2021 6,487 10 View