PGEM®-11Zf(+) has two promoter (T7 and SP6) in it, where can I insert my chimeric vaccine construct for a in vitro transcription approach?

I want to insert a designed self-amplifying mRNA vaccine construct [containing (i) genes encoding non-structural proteins (i.e., nsP1-4) of an alphavirus and (ii) genes encoding targeted epitopes]...

14 June 2020 3,753 4 View